Gonadotoxic Potentials of Di-(2-Ethyl Hexyl) Phthalate in the Adult Male Wistar Rats
World health organization (WHO) estimates a global infertility prevalence rate of 10-15%, and 20-30% among Nigerians. Male factor constitutes about 40-50% of infertility cases. Phthalates as environmental pollutants (EPs) adversely affect differentiation and functions of reproductive system with intrauterine or neonatal exposures, predisposing to infertility later in life. This study, therefore, aimed at investigating the effect of oral di-(2-ethylhexyl) phthalate (DEHP) on some gonadal functions and testicular histology in adult Wistar rats. Fourty (40) adult male Wistar rats weighing between 156-250g were randomised into 4 experimental groups A, B, C and D (n=10/group) and were orally treated daily with 0.02mg, 20mg, 200mg DEHP/kg respectively for 60 days. Fasting samples of semen, serum and testicular tissue were obtained for analysis on day 61. Results obtained were expressed as mean ± standard deviation and statistical analysis was done using one way Anova to test for significant differences in means. The results showed that relative to the control reference values, sperm count, total and active sperm motility, normal sperm morphology and serum testosterone concentration were significantly (p<0.05) reduced while sluggish sperm motility and abnormal morphology showed significant (p˂0.01) increases. Testicular tissue showed features of necrosis with abnormal cellular differentiation and aggregation, with haemorrhagic spots. The study has shown that DEHP induces gonadotoxicity in adult male rats, thereby potentiating male infertility. This implies that EPs are hazardous to reproductive health and hence, the need to reduce exposure to them, thereby substantially preventing male infertility
Copyright (c) 2019 SA Ogli, SO Odeh, GU Egesie
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